Antitumor effect of IL-12 gene-modified bone marrow mesenchymal stem cells combined with Fuzheng Yiliu decoction in an in vivo glioma nude mouse model

Abstract Background Glioma is a complex cancer with high morbidity and mortality. Bone marrow mesenchymal stem cells (BMSCs) have shown promise as an excellent cell/drug delivery vehicle for gene-targeted therapy; however, maintaining genetic stability biological activity remains difficult. Furthermore, whether BMSCs support or inhibit tumor growth debated. This study investigated traditional Chinese medicine fomular, Fuzheng Yiliu decoction (FYD) had synergistic antitumor effect IL-12 gene-modified in glioma-bearing nude mice Methods The lentivirus-mediated gene was transfected into primarily cultured BMSCs. A total of 72 BALB/c were used to establish xenograft models glioma U251 divided groups (n = 12) including blank control group, mouse model group (model group), lentiviral transfection BMSC no loading (BMSC lentivirus-transfected (IL-12 + FYD treatment (FYD group).. After 14 days, all sacrificed collect tissue serum more detection, such distribution BMSCs, cell apoptosis tumors, INF-? levels, weight volume measured Results There significantly apoptotic combining than that the ( P < 0.05). showed higher Bax lower Bcl-2 expression 0.05), levels 0.05) other groups. intervention, this also strong inhibitory against Conclusions suggested combined shows mice.